To reflect this progression, the chapters in our Sequence Data Analysis Guidebook are arranged, not by software package, but by fimction. It is now possible to progress from isolate d DNA to database submission without writing a single base down. Much of this soft ware may now be used on relatively inexpensive computers. It can assemble, align, or compare multiple fragments, perform restriction analyses, identify coding regions and specific motifs, and even design the primers needed to extend the sequencing. Computer software is now available that can run gels, collect data, and assess its accuracy. This book reflects that change since the majority of packages described here are designed to be used on desktop computers. Computers have become smaller and more powerful and the software has become simpler to use as it has grown in sophistication. Recently their role has taken a further major step forward. It is unlikely that any sequencing projects have been performed in the last few years without the aid of computers. Computers have revolutionized the analysis of sequencing data.
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